Detection of somaclonal variation in plants regenerated from different tissues of strawberry (Fragaria x ananassa) using ISSR marker
Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:

Production of genetically and phenotypically stable plantlets is the main purpose in commercial strawberry tissue culture. In this study, different tissues of Fragaria ananassa cv. Camarosa including stipule, apical meristem, leaf and petiole were cultured in Murashige and Skoog (MS) medium supplemented with different concentrations of N6-benzyladenine (BA) (0.5, 1, 2, 3 mg/L) and indole-3-butyric acid (IBA) (0.1 and 0.5 mg/L). For apical meristem explants, the best regeneration rate (7.6 shoots per each explant) was obtained in the medium containing 1 mg/L BA and 0.1 mg/L IBA. Whereas stipule explants showed the highest regeneration rate in the medium containing 2 mg/L BA and 0.1 mg/L IBA. For leaf and petiole explants, the medium containing 2 mg/L BA and 0.5 mg/L IBA had the best hormonal combination. To determine the genetic variation, micropropagated plants from different tissue (after 8 subcultures) were analyzed by the inter-simple sequence repeats (ISSR) molecular marker. Among the 18 pre-selected primers, 10 displayed clear, reproducible and informative bands. A total of 88 distinct bands with a polymorphic rate of 46% were produced in the molecular profile of different explants. The highest and the lowest similarity values to maternal plants belonged to stipule and petiole explants with a similarity index of 0.738 and 0.645, respectively. Vitroplants derived from stipule and apical meristem showed the highest genetic stability with respect to maternal plants. With respect to genetic stability and regeneration rate, apical meristem can be recommended as suitable explants with the highest genetic fidelity. The findings of this study could be applied for commercial scale multiplication of strawberry, and also demonstrate that ISSR markers are eligible for detection of somaclonal variations.

Language:
English
Published:
Iranian Journal of Genetics and Plant Breeding, Volume:7 Issue: 2, Oct 2018
Pages:
65 to 72
magiran.com/p2049950  
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 990,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
دسترسی سراسری کاربران دانشگاه پیام نور!
اعضای هیئت علمی و دانشجویان دانشگاه پیام نور در سراسر کشور، در صورت ثبت نام با ایمیل دانشگاهی، تا پایان فروردین ماه 1403 به مقالات سایت دسترسی خواهند داشت!
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 50 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!