Comparison of the effect of construction and cultivating of Stevia rebaudiana Bertoni specimens on TIS bioreactor in comparison with in vitro culture

Due to self-incompatibility, the high seeding of seeds and the lack of uniformity of the plants in terms of seedling quality and macrobiotics content of the stevia herb, through novel tissue culture methods, is a suitable strategy for the massive cultivation of this medicinal plant. In this study, in order to investigate the growth parameters differentiation, the stevia plant specimens were compared in in vitro culture and TIS bioreactors. Due to the possibility of co2 exchange on top of the TIS bioreactors and the exchange of nutrients due to the intermittent operation of the pumps, they can be one of the most important reasons for the superiority of the TIS bioreactor activity compared to in vitro culture. Due to the significant decrease in yield and growth rate of samples in in vitro culture, due to the lack of exchange of nutrients, the culture medium due to the use of agar in solid culture can be carried out using TIS bioreactor culture medium and increased the growth rate significantly. Also, the culture of the samples in the TIS bioreactor, due to the appropriate volume of archers and the temporary flood conditions of the culture medium, can allow the yield of the explants under relatively similar conditions, so that the possibility of reducing the seedlings and the subsequent contamination Transmission also declined. Non-agar use and reduction of particle retention. This method can be used to produce massive stevia seedlings with the highest yield.