Determination of H2O2 in Human Serum Samples with Novel Electrochemical Sensor Based on V2O5/VO2 Nanostructures

Highly selective and sensitive analysis of hydrogen peroxide (H2O2) has attracted considerable interest in the fields of clinical diagnostics, food industry, and environmental analysis.

In the present study, an efficient electrochemical sensor, based on V2O5/VO2 nanostructures, was introduced for measuring hydrogen peroxide (H2O2) in human serum samples.

The characterization of the prepared V2O5/VO2 nanostructures was investigated by X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDX). A carbon paste electrode (CPE) modified with V2O5/VO2 was applied for the electrochemical detection of H2O2.

The prepared sensor depicted a good linear range from 8 to 215 μM and a low detection limit of 5 μM. Moreover, the modified electrode showed notable anti-interference property and high sensitivity toward H2O2 detection. The suggested method was also successfully applied for the determination of H2O2 in human serum samples.

The V2O5/VO2 embedded CPE offers good simplicity, sensitivity, and selectivity toward H2O2 determination. In addition, the suggested assay revealed good reproducibility and anti-interference property in the measuring of H2O2.