The effect of exogenous glycinebetaine on proline and salt tolerance of transgenic tobacco (Nicotiana tabacum) plant under in vitro culture
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Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
In the present study, transgenic tobacco (Nicotiana tabacum) plants over expressing P5CS gene under in vitro salt stress condition. Tobacco plants transferred to MS medium containing 100 and 200 mM NaCl, then foliar application of two glycine betaine concentrations, including 20 and 40 mg L-1 were applied on the surface of the plants with four to six leaves at University of Isfahan in 2015 with a completely randomized design with three replications. After 4 weeks post treatment results showed that, exogenous glycine betaine under salt stress increased fresh and dry weight, K+, content, leaf area, endogenous glycine betaine and soluble sugar. In contrast, the amount of photosynthetic pigments, proline, content, Na+ content, and Na+/K+ ratio were decreased. The results of this study showed that, exogenous application of glycine betaine in tobacco plants was effective in reducing the negative effects of salt stress and improved growth parameters. However preliminary experiments showed a decrease of proline and increase of endogenous glycine betaine in stressed platns. On the other hand, results indicated that, communion cooperation of these osmolytes to cope with stress conditions has possibly been occurred. According to the results of this study, that cooperative roles of glycine betaine and proline in membrane stability and reducing the negative effects of salinity in both transgenic and non-transgenic plants it can be suggested.
Language:
Persian
Published:
Journal of Plant Research, Volume:32 Issue: 4, 2020
Pages:
815 to 825
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