Screening heavy metal resistant halotolerant strains producing non-ribosomal peptides

Todays, secondary metabolites from microorganisms have been to improve food, pharmaceutical and chemical industries . Extremophile microorganisms are one of the resources for the secondary metabolites. The heavy metal resistant halotolerants were isolated from Haj AliGholi Khan Salt Lake. The potential of the isolates for producing secondary metabolites were molecularly investigated by amplification of nrpS genes with specific primers A3/A7 and NS1/NS2. In the screening of Haj AliGholi Khan Lake microorganisms; 13% of isolates from MGM, 19.5% from MH, 43.75% from SWN, 3.7% from LNSWN and 20.62% from normal Nutrient agar were obtained. The halotolerant were highly resistant against Ni and highly susceptible to Cd. Three to 7 PCR products were identified from optimized PCR reaction of nrpS genes of heavy metal resistant halotolerant with specific primers. Existence of nrpS genes among halotolerant and semi- halophile microorganisms from Haj AliGholi Khan Salt Lake revealed a direct relation between heavy metal resistance and genetic potential for producing secondary metabolites.