In-vitro Apoptotic Effects of Deferoxamine on the Glioblastoma Cell Line
Message:
Abstract:
Background

Research suggests the inhibitory effects of deferoxamine as an iron chelator on erythroleukemia cells. The present study was conducted to investigate the effects of deferoxamine on B92 cells as a model of glial cells carcinoma.

Materials and Methods

The present experimental study treated 6×104 B92 cells with 0, 10, 50 and 100 µM of deferoxamine for 24 hours in the presence or absence of 10 μmol/l of ferric chloride. Morphological changes were evaluated in the treated cells compared to in the control sample using an inverse optical microscope. The inhibitory and cytotoxic effects of deferoxamine were evaluated using the dimethylimidazole-diphenyl tetrazolium bromide (MTT) reduction and neutral red uptake assay. The data were analyzed using the Kruskal-Wallis test. P <0.05 was set as the level of statistical significance.

Results

The inhibitory effects of deferoxamine on the proliferation of B92 cells were identified after 24 hours in a way that the cells began to accumulate in the presence of deferoxamine. Ten μmol/l of ferric chloride prevented these morphological changes. Deferoxamine was also found to significantly and 
dose-dependently inhibit the vitality and viability of B92 cells. Moreover, the data showed that ferric chloride prevents the emergence of the effects of treating B92 cells with deferoxamine.

Conclusion

Deferoxamine exerts in-vitro antiproliferative effects on the glial cell line B92.

Article Type:
Research/Original Article
Language:
Persian
Published:
Iranian South Medical Journal, Volume:22 Issue:5, 2019
Pages:
264 - 277
magiran.com/p2051744  
روش‌های دسترسی به متن این مطلب
اشتراک شخصی
در سایت عضو شوید و هزینه اشتراک یک‌ساله سایت به مبلغ 300,000ريال را پرداخت کنید. همزمان با برقراری دوره اشتراک بسته دانلود 100 مطلب نیز برای شما فعال خواهد شد!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی همه کاربران به متن مطالب خریداری نمایند!