The Effect of Plant Growth Regulators on Micropropagation and Regeneration of Thymus Daenensis Celak

Daenensis thyme (Thymus daenensis Celak) is one of the important medicinal plant that belongs to the family Labiatae. The leaves and flowering branches of this plant has a number of important compounds such as Thymol, Carvacrol, Btaptyn and Parasymyn. The present study was performed in order to determine the optimum of plant growth regulators (auxin and cytokinin) for production of in vitro plantlet using apical bud and hypocotyl explants. The seeds after sterilization were germinated on MS medium. The explants were cultured on the medium containing various concentrations of BAP and Kin for study of in vitro micropropagation. Hypocotyl explants were cultured on the medium containing various combinations of BAP and NAA for callus induction and shoot regeneration. In study of micropropagation, the mean comparison showed the highest number of shoot, leaf and bud obtained in MS medium containing 2.5 mg l-1 Kin in the apical explants. In study of regeneration, the mean comparison showed the highest percentage of shoot regeneration and mean of shoot number were obtained on MS medium supplemented with 1.5 mg l-1 BAP + 0.6 mg l-1 NAA in hypocotyl explants. Overall, Kin was most effective plant growth regulator on micropropagation and comination of BAP and NAA had most effect on regeneration of Daenensis thyme.