Antimicrobial Activity of Streptomyces Tendae Strain 944 Isolated from the Eastern Regions of Gilan Province and Optimization of its Bioactive Compounds
Streptomyces are microorganisms that are present in a variety of natural resources, especially in the soil, and can produce a wide variety of antimicrobial compounds. The prevalence of microbial resistance among pathogen microorganisms is increasing. The purpose of this study was to isolate and identify Streptomyces producing antimicrobial compounds with molecular methods and optimize its production conditions.
Streptomyces isolated from the soils of different regions of eastern Gilan province. Then, morphology, physiology and biochemical identification of the isolated samples were investigated. Finally, the identification of the isolates was carried out using sequencing of 16 SrRNA and phylogenetic analysis. Antimicrobial activity against tested pathogenic microorganisms and optimization of antimicrobial compounds produced in nutrient media and different conditions such as carbon and nitrogen sources, pH, incubation time and temperature were performed.
Based on the results of phylogenetic studies and sequencing of 16SrRNA, 97.34% confidence was detected in Streptomyces tendae strain 944. The bacterium had antimicrobial activity against pathogenic microorganisms including Micrococcus luteus PTCC 1408, Bacillus cereus PTCC 1154, Staphylococcus aureus PTCC 1189, Pseudomonas aeruginosa PTCC 1310, Salmonella typhi PTCC 1609 and Proteus mirabilis PTCC 1776. After optimization, the best culture medium, pH, optimum temperature, carbon and nitrogen sources and incubation time were ISP2, 7, 28 0 C, glucose, yeast extract and 7 days respectively.
The first report of screening of Streptomyces producing bioactive compounds in the eastern regions of Guilan province, show that the soils of the eastern regions of Guilan province are a very rich source of streptomyces producing antimicrobial compounds that can it is very important to produce natural-antimicrobial compounds.
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