Study on the effect of low doses of gamma irradiation alone or in associated with the salicylic acid and indirect temperature on seed borne infection of Bean common mosaic virus in bean plant

In this study, the effect of the low doses of gamma irradiation (10, 20, and 30 Gy) alone and in associated with the salicylic acid and indirect temperature on the seed borne infection of Bean common mosaic virus</em> (BCMV) was assessed. Commercial bean seeds called “Sadri” with seed borne infection to BCMV were sowed. The seedlings were tested for the infection of BCMV by indirect plate-trapped antigen (PTA- ELISA) test and seeded. Results indicated that the growth factors in the irradiated seeds alone and in associated with the salicylic acid or indirect temperature significantly increased (P≤0.05) in healthy and infected seedlings compared to the non-irradiated seedlings. Gamma irradiation induced the specific activity of various antioxidant enzymes (PPO, POX, CAT and PAL) as well as the total protein density in treated healthy and infected beans, and decreased the concentration of BCMV proteins in the infected and irradiated seeds alone and so in associated with the salicylic acid or indirect temperature compared to the non-irradiated seedlings. Results of this research indicated to the induction of the relative resistance against BCMV in infected bean seedlings treated with the 20 and 30 Gy doses of gamma irradiation alone or in associated with the salicylic acid and indirect temperature.