Identification of native isolation, FUM1, isolated from poultry farm of Mashhad countryside and improving its keratinolytic activity by optimizing of environmental conditions using one factor at a time methodology

Significant amounts of waste, including feathers, bones, blood etc. are yearly produced by the poultry industry. Feathers are composed of 90% keratin protein, and the rest is composed of lipids and water. Keratinases are one of the most diverse and usable enzymes which can be produced by bacterial and fungal microorganisms. These enzymes show a wide range of application in the various field. In this study, the keratinolytic activity of the isolated strain from a poultry farm in Mashhad was evaluated and then the medium conditions for keratinase production were optimized. The strains were identified based on the morphological and biochemical methods. 16S rRNA gene of the strain was amplified by PCR and then sequenced. And the strain proteolytic activity was examined and compared with its keratinolytic activity. Finally, strain growth ability tested in variety substrate. using 16SrRNA gene sequencing, morphological and biochemical identification, the strain shared 99/9% similarity with Bacillus mojavensis. Optimization of various factors including temperature, pH, incubation time, carbon and nitrogen sources, aeration and inoculum size showed that the isolated strain has the highest keratinolytic activity at 37°C, 48 hours incubation period, pH=9/5, sucrose 1%, 3% substrate, aeration 75% and 6% (v/v) inoculum amount. None of the nitrogen sources had a positive effect. the FUM-1 keratinolytic activity was increased approximately 3.38 fold by condition optimization of the medium, indicating the importance of environmental conditions. In the study, the strain with high keratinolytic activity was suggesting its potential use in biotechnological.