Detection of G276T Polymorphism from Adiponectin Gene Using PCR-RFLP Technique in Patients with Breast Cancer
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Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Introduction

Adiponectin hormone is involved in modulating metabolic processes such as glucose regulation and fatty acid catabolism. Several single nucleotide polymorphisms (SNPs) in the transcription region of the gene and its sequences have been identified in different populations that are associated with the prevalence of various diseases. In this study, PCR-RFLP technique was used to identify G276T polymorphism in patients with breast cancer.

Material and Methods

128 individuals with breast cancer and 128 healthy people were selected after the approval of a specialist physician. After blood collection and DNA extraction, all specimens were amplified with specific primers, then part of them was cut with Mva1269I enzyme and the genotypes of individuals were determined. The results were analyzed using SPSS version 23 software by chi-square and logistic regression tests.

Results

The two groups did not have a significant difference for mean age and body mass index. TT and GT genotypes were significantly different in the two groups and increased the risk of breast cancer by 2.987 and 1.489 in their carriers, respectively. GG and GT genotypes showed a significant relationship with age and body mass index.

Conclusion

The results showed a strong association between adiponectin G276T polymorphism and breast cancer. Carriers of the heterozygote GT genotype were more likely to develop breast cancer with increasing age and body mass index.

Language:
Persian
Published:
Pars Journal of Medical Sciences, Volume:17 Issue: 2, 2019
Pages:
8 to 14
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