Effect of Phosphate-Buffered Saline on the Push-Out Bond Strength of Mineral Trioxide Aggregate in the Presence of Blood in the Simulated Furcation Perforations

Dentin bond strength of the perforation repair materials is one of the effective factors on the materials sealing ability and success of the treatment. The aim of this study was to evaluate the effect of phosphate-buffered saline (PBS) on the push-out bond strength of White Mineral Trioxide Aggregate (WMTA) in the presence of blood contamination in simulated furcation perforations. This study was performed in Dental and Periodontal Disease Research Center in Tabriz Dental Faculty with the ethics code of IR.TBZMED.REC.B1396.819 in 2017. The furcation perforation measured with 1.3 mm diameter and 2 mm height was created in the 60 mandibular molars, and then the specimens were divided into four groups of 15 samples. In groups 1 and 3, only irrigation with normal saline after perforation establishment was done. In groups 2 and 4, blood contamination was created in the walls of perforation. Afterward, one Gel tamp was placed under the perforations in all the teeth, and WMTA was placed in the perforations. The moistened cotton with normal saline was placed on the MTA in groups 1 and 4, and moistened cotton with PBS was placed on the MTA in groups 2 and 3. Instron test was done after 1 week using universal testing machine. Due to the normal distribution of the samples, the two-way analysis of variance was used for statistical analysis. P-value less than 0.05 was considered statistically significant. In the presence and absence of blood contamination, the mean bond strength of WMTA in saline proximity was significantly higher than PBS (P<0.05). The blood contamination caused to significantly decrease the bond strength (P<0.05). The presence of PBS does not have any positive effect on the increase of the bond strength of WMTA.