The Effects of High Intensity Interval (HIT) vs. Continuous Trainings on ABCG5 and ABCG8 Genes Expression in Male Wistar Rats after High Fat Diet

ABCG5 and ABCG8 are two membrane cholesterol transporters involved in transportation of this substance across the membranes of hepatocytes and enterocytes. The aim of the present study was to investigate the effects of continuous and interval trainings on gene expression of these substances in hepatocytes of male wistar rats.
Methods and Materials: In the current experimental study, 15 rats were assigned into groups of control with high fat diet (group 1, N=5), interval training with high fat diet (group 2, N=5), and continuous training with high fat diet (group 3, N=5). Duration of trainings was 12 weeks (5 days/week). Mann-Whitney U test was used for statistical analysis of data. 

The results showed that ABCG5 and ABCG8 genes expression after trainings were higher in groups of 2, 3, and 1, respectively. As for ABCG5, differences between groups 1 vs 2, 1 vs 3, and 2 vs 3 and for ABCG8, differences between groups 1 vs 2 and 2 vs 3 were significant (P=0.008) and only the difference between  groups 1 vs 3 regarding ABCG8 was non-significant (P=0.841).

It seems that exercise trainings are more effective on ABCG5, as compared with ABCG8 gene expression, and HIT is more effective compared with continuous trainings, on liver cholesterol excretion during reverse cholesterol transport.