Bad gene expression following effect of coenzyme Q10 on Wistar rat hippocampus with cerebral ischemia

Q10 coenzyme is a potent antioxidant in the mitochondrial membrane. Releasing the oxygen free radicals occurs in the cerebral ischemia. Using Q10 coenzyme causes strength against oxidative after injury of cerebral ischemia during reperfusion. Also CoQ10 plays an important anti-apoptotic role to reduce Caspase 3 as a key enzyme neuroprotective in apoptosis. According to the sensitive nature of CA1-Hypocampus pyramidal neurons against the cerebral ischemia, we studied the effects of CoQ10 in this area of rat`s brain after transient general ischemia/ reperfusion.

We selected randomly 24 heads of Wistar rats under 250-300 g weights that divided into 4 groups, including control, experiment (ischemia, Q10), ischemia and Q10 coenzyme groups. Then we separated the hippocampus from the brain expression of pro-apoptosis genes was studied. After extraction of RNA and synthesis of cDNA, we used real time RCR methods to express this gene.

The expression of Bad gene in experiment group (Q10, 10mg/Kg) significantly decreased compared to ischemia group (p<0.05).

It seems that ischemia caused to increase the expression of Bad pro-apoptic gene, and CoQ10 with dose of 10mg/Kg has effects on the expression of Bad gene and prevent the severity of lesions.