n order to investigate the effect of in ovo injection and utilization of different levels of licorice extract (LE) (Glycyrrhiza glabra) and colloidal nano silver (CNS) on performance and some blood parameters of broilers during embryonic and growth periods as substitution for Flavomycin®, an experiment was conducted using eight hundred Ross-308 Fertilized eggs as factorial with completely randomized design with 10 treatments and 4 replicates of 20 eggs in each.The treatments of in ovo injection stage consisted of LE (0, 100 and 200ppm) and CNS (0, 30 and 60ppm), negative control group (without injection) and positive control group (injection of 0.1ml sterilized distilled water).The treatments of growth period consisted of LE (0, 1 and 2gr/kg) and CNS (0 and 120ppm), treatment containing 450 ppm antibiotic Flacomycin® and two control group from embryonic period.The results showed that the injection of 100ppm LE+ 60ppm CNS significantly increased hatchability percentage(P<0.01) when compared with groups treated with LE and CNS.The chickens body weight and length after hatch were increased significantly in all treatment especially in treatment containing the highest levels of CNS and LE (P<0.01).The highest FCR was observed in group treated with 450mg/kg antibiotic Flacomycin® and negative control(P<0.01) and the lowest FCR was observed in treatment containing 120 mg/kg CNS and treatment containing 2gr/kg LE+ 120mg/kg CNS(P<0.01).The HDL concentration was increased by treatment containing 100ppm LE(P<0/05).Generally, licorice and colloidal nano silver could improve performance and carcass characters via upgrade physical conditions of chicken and can be considered as a substitute for antibiotic Flavomycin.
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