The effect of high-intensity interval training (HIIT) on SYD and MuRF1 gene expression of in diabetic rats

Syd and Murf1 are important molecular markers for muscle atrophy that increase significantly in skeletal muscle in various conditions, such as diabetes. The aim of this study was to evaluate the effect of high-intensity interval training (HIIT) on syd and murf1 genes expression in skeletal muscle of diabetic rats.

To the implementation of this experimental research, 60 male Wistar rats weighing 220 ± 20 gr randomly were divided into 5 groups including baseline control, eight-week control, diabetes, training, and diabetes-training. In this study, the rats were diabetic using given a single dose of 50 mg/ kg per body weight and peritoneal injection streptozotocin. High-intensity interval training performed on the treadmill with intensity of 80-85% VO2max, 5 days a week and for 8 weeks. The expression of syd and murf1 genes were measured by Real-Time PCR. Data were analyzed by one-way ANOVA and Bonferroni's post hoc test at the p<0.05.

The changes in the expression of Murf1 and Syd genes of the skeletal muscle in the diabetes group were significantly higher than the control group (P=0.001). The high-intensity interval training significantly reduced the expression of Murf1 and Syd genes (P=0.001).

HIIT may help reduce atrophy in diabetic patients by reducing the expression of syd and murf1 genes in skeletal muscle.