The Mediating role of Resilience in the Relationship between Self-esteem and Self-compassion with Psychological Well-being of Military Persons
Author(s):
Message:
Abstract:
Aims

Today, one of the most important problems in detection of human pathogens, is lack of positive control. The idea of using hybrid vectors, containing genes of different pathogens, can overcome this limitation. We can design specific primers for each region and use the hybrid vector as positive control sample in PCR. In this research we designed an in silico hybrid vector and relevant primers for detection of Francisella, Variola, Burkholderia and Yersinia.

Materials & Methods

In this study, fopA, caf1, 16srRNA and HA genes were chosen to be located on the vector, to respectively represent Francisella, Yersinia, Burkholderia and Variolla,. The sequence of these genes were obtained from NCBI in FASTA format and were aligned in BioEdit 7.0.5.3 software for finding conserve region of each gene, then some purposeful changes were applied in the sequence of each gene and the sequences were placed next to each other and the construct was designed. Specific primers were designed for each region using Oligo7, BioEdit, OligoAnalyzer tool as well as NCBI database. Finally, the construct was cloned in PUC57 in SnapGene 3.2.0.1 and PCR was simulated on hybrid vector using designed primers.

Findings

Analysis confirmed that conserved regions for each gene were located on hybrid vector, and simulation of PCR proved the accuracy of designed primers.

Conclusion

A genetic simulator hybrid construct with the types of primers required to identify the four factors Francisla, Varivola, Borghuleria and Yersinia has been designed in silico so that such factors could be identified under positive control in emergencies.

Language:
Persian
Published:
Journal of Police Medicine, Volume:9 Issue:1, 2019
Pages:
17 - 26
magiran.com/p2070829  
روش‌های دسترسی به متن این مطلب
اشتراک شخصی
در سایت عضو شوید و هزینه اشتراک یک‌ساله سایت به مبلغ 300,000ريال را پرداخت کنید. همزمان با برقراری دوره اشتراک بسته دانلود 100 مطلب نیز برای شما فعال خواهد شد!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی همه کاربران به متن مطالب خریداری نمایند!