Investigating the possibility of removing egg adhesion by the alkaline, tannic acid and sodium chloride enzymes instead of carbamide solution in the artificial propagation process of common carp (Cyprinus carpio)

In this research, in order to eliminate the adhesion of fertilized eggs, common carp of common carp from three materials of alkalase, tannic acid and sodium chloride was used. The results of fertilization rate and hatching rate were compared with the general method of the workshop (egg washing with carbamide solution). 9 concentrations of sodium chloride were used at 25, 50, 100, 250, 500, 1000, 2000, 4000 and 10000 mg / L for three times 15, 30 and 60 minutes. Three concentrations of tannic acid (500, 1000 and 1500 mg / L) were used at three times 0.5, 2 and 5 minutes. All three concentrations were performed in three replicates. After activation, 50 milliliters of the enzyme were added at a concentration of 2 milli liters per liter for 20 minutes and exposed at 45, 60, 75, and 90 seconds. Finally, they were washed 4 times with hatchery juice and transferred to incubation. The results showed that the best fertilization rate (99.57%) and hatching rate (99.46%) were related to treatment 1 at a concentration of 500 mg / L in 0.5 time It was a minute. Regarding removal of egg adhesion by sodium chloride treatment, the best fertilization rate (87.1) and hatching rate (28.86) were related to 1000 mg / l treatment at 30 minutes. The highest percentage of fertilization (97.52%) and hatching (96.72%) with alkaline enzyme was 50 mg / L treatment at 45 seconds.