Effects of dietary black cumin seed on haemolymph biochemical parameters of Pacific white shrimp (Penaeus vannamei)

This study was designed to determine the effects of  different levels of dietary black cumin seed on some haemolymph biochemical parameters in pacific white shrimp, penaeus vannamei. Five levels of black cumin seed including 0, 0/5, 1/5, 3 and 5 percent were used and each group was divided in three replicates and 40 shrimp (7.5±0.2 gr) were stocked in each replicate. After 12 weeks of feeding trial, plasma glucose, alkaline phosphatase (ALP) and superoxide dismutase (SOD), levels were significantly lower in the shrimp fed diets contained black cumin seed compared to the control (P<0.05). Moreover, plasma creatinine (CREA), total protein, hemolymph urea nitrogen (HUN), uric acid and urea were numerically lower in shrimp fed diets contained black cumin seed compared to the control (P>0.05). Calcium and magnesium were not significantly different among the treatments (P>0.05). In addition; phosphate increased significantly in the groups fed diet contained 1/5 and 5 percent black cumin seed when compared to the control group (P<0.05). In conclusion, this study indicated that dietary black cumin seed, have impressive effects on shrimp haemolymph biochemical parameters and can help to produce shrimp permanently.