Morphological and molecular identification of sea cucumber dominant specie in Ouly marine area (Persian Gulf- Bushehr province) and analyzing of its certain body-wall compositions

This research aimed to analyze some of body compositions also collagen protein, in one of dominant species of sea cucumbers in Ouly-Bushehr area, with species morphological and molecular identification. may be with this study, in addition of exact species Identification, our finding in case of its body wall compounants determination, help to more improvement of medical and nutritional industries in our country,Iran. the result of morphological identification according to body wall ossicles shape and then by molecular identification according to partial sequencing of 16SrRNA mitochondrial DNA indicated the species is “Holothuria leucospilota”. In case of body composions analyzing, protein and moisture were two aboundant compounants with more than 70% in amount and fat was the fewest one. Among protein compounants, collagen was extracted by pepsinal enzymatic hydrolyzing. The type of extracted collagen protein and its subunite composition was determined by SDS-PAGE method. As a result, the extraction method was effective and applicable and the collagen was type (I) with three α1 chains had about 140 kDa molecular weight.