Effect of Hydroalcoholic Extract of Jujube Fruit (Zizyphus jujube L.) in Rats with Polycystic Ovary Syndrome

Polycystic ovary syndrome is one of the most important endocrine disorders affecting about 5-10% of women in reproductive age. Some herbs are able to prevent or inhibit the development of syndrome. The aim of this study was to determine and effect of hydroalcoholic extract Zizyphus jujube L. fruit's in rats with polycystic ovary syndrome.

In the present experimental study, 42 immature female Wistar rats, aged 3-4 weeks, weighing 50-70 g were obtained from the Animal Husbandry Center of Hamadan University of Medical Sciences. The rats were randomly divided into seven equal groups; Treated with metformin and treatment 1, 2 and 3 were randomly divided. Control group received 0.5 ml of normal saline intraperitoneally. The control group received 250 mg / kg testosterone enanthate dissolved in sesame oil in a subcutaneous injection in the back of the neck. The sham group received 0.5 ml of sesame oil in subcutaneous injection. The metformin-treated group received 250 mg / kg subcutaneous testosterone enantate plus metformin 500 mg / kg by gavage. Treatment groups 1 and 2 received 250 mg / kg testosterone enantate and jujube hydroalcoholic extract at doses of 400 and 800 mg / kg, respectively, for one month. Treatment group 3 received testosterone enantate for 4 weeks and after 4 weeks, they received intraperitoneal injection of jujube extract at a dose of 800 mg / kg for one week. At the end of the experiments, the animals were first anesthetized with ether and tissue samples of the ovaries were prepared for histological examination and H&E staining. Collected data were analyzed using one-way ANOVA, Kruskal-Wallis, Kolmogorov-Smirnov and Tukey post hoc tests.

The results of the present study indicated that testosterone enanthate produced polycystic follicle in rat's ovary. A significant decrease was seen in polycystic ovary in treatment groups with ZHE compared with the witness group (P<0.001).

Zizyphus jujube L is likely improved the polycystic ovary syndrome due to its specific chemicals and compounds such as ascorbic acid, flavonoids, vitamins A and C and calcium