In order to evaluate the effect of different bio-fertilizer on agronomical traits and photosynthesis pigments of linseed under irrigation cut-off condition a farm experiment carried out as split plot base on randomized complete block design with three replication at research field of Yasouj university in 2017. Irrigation regimes at three levels, full irrigation, irrigation cut-off at beginning of flowering until beginning of podding, irrigation cut-off at beginning of podding to end of maturity and bio-fertilizer application at four levels as mycorrhiza arbuscular fungi, Phosphate Barvar2, mycorrhiza arbuscular fungi+Phosphate Barvar2 and non-application of bio-fertilizer were considered as the main and the sub plots, respectively. Results showed that the lowest grain and oil yield (93.7 and 32.9 g.m-2) and the lowest  capsule numbers per plant, seed numbers per capsule and 1000 seed weight and also the highest seed protein percent were achieved from irrigation cut-off at  beginning of podding to end of maturity. Application of mycorrhiza arbuscular fungi + Phosphate Barvar2 comparison with non-application of bio-fertilizer increased capsule numbers per plant, grain and oil yield, and protein percent by 25.5, 26.5, 33.1 and 17.4 percent respectively. Use of mycorrhiza arbuscular fungi + Phosphate Barvar2 under irrigation cut-off at beginning of podding to end of maturity increased leaf carotenoid content significantly in seed filling stage sampling. Also in full irrigation condition at beginning pooding stage sampling, Use of mycorrhiza arbuscular fungi + Phosphate Barvar2 increased leaf chlorophyll content significantly.