Acid humic effect on germination indices and antioxidant enzymes of rapeseed (Brassica napus L.) seeds under the influence of toxicity of copper oxide nanoparticles

Due to the increasing production of nanoparticles in recent years and their applications in various applications such as medicine, imaging, catalysts, semiconductors, cosmetics, microelectronics, coatings on various materials, etc., there are currently concerns about the potential hazard and environmental pollution. Nanoparticles have been created globally. The purpose of this study was to investigate the effect of copper oxide nanoparticle toxicity on the activity of some antioxidant enzymes, protein and germination indices of canola, and also to investigate the effects of humic acid on nanoparticle toxicity in laboratory environment. An experiment was conducted at the germination stage of rapeseed (Octopus cultivar) to investigate the effect of copper oxide nanoparticles in concentrations of 0, 250, 500, 750, 1000, 1250, 1500, 1750, 2000 mg per liter and humic acid in concentrations of 0, 100 mg / L, in 3 replicates, a factorial in a completely randomized design. The results showed that, with increasing concentrations of copper oxide nanoparticles, the levels of activity of all four superoxide dismutase enzymes, ascorbate peroxidase, catalase and peroxidase increased (5.00, 2.9, 2.00, 2.35), respectively, but the amount of protein 2 times lower. Germination indices (root length, stem length) also decreased (39.3, 56.6, 12.7), respectively. The use of humic acid reduced the toxicity effects of copper oxide nanoparticles, especially at the level of 2000 mg / L, so that the root length, stem, root and shoot stomatal length (respectively) 68/9, 0/3, 0/6, 5/5) decreased compared to the control. It can be stated that humic acid can increase the activity of antioxidant enzymes and increase growth by increasing the activity of quasi-hormonal activity.