Effect of UV-A and UV-B radiation on the production of rosmarinic acid and caffeic acid in callus culture of Salvia leriifolia Benth.

Salvia leriifolia Benth. is one of the most important medicinal plant so, the purpose of the present study is firstly improve callus production with root and leaf explants in combination of NAA and 2.4-D with Kin in MS medium. The results showed that NAA was not produced callus in the leaf and root explant, while with 2.4-D, the most suitable callus was obtained from the leaf explant at a concentration of 1 mg/ lit Kin with 2 mg/ lit 2,4-D. Then, effect of UV-A and UV-B radiation as elicitors in two times (30 and 60 min) on content of phenolic, flavonoid, rosmarinic acid and caffeic acid in callus of Norouzk plant was investigated. The results showed that UV-A and UV-B radation increased content of total phenol, flavonoids, rosmarine acid and caffeic acid in callus. The highest fresh weight, phenol and flavonoid content were observed in 30 minutes UV-A treatment and the highest accumulation of rosmarinic acid and caffeic acid was observed in the calluses treated with 30 minutes UV-B radiation. Therefore, by optimizing the uv ellicetor, it is possible to produce the desired secondary metabolites like as caffeic acid of salvia in callus culture.