The Effect of Magnesium Sulfate on Sperm Characteristics and Blood Hematology After Testicular IschemiaReperfusion Injury in Rats

The aim of this study was to investigate the effect of magnesium sulfate (MgSO4) administered single intraperitoneally on sperm characteristics after testicular ischemia-reperfusion (IR) injury in the male rats.

Thirty adult rats were randomly divided into 5 groups: sham operations (group 1), IR (group 2), IR + 125 mg/kg MgSO4 (group 3), IR + 250 mg/kg MgSO4 (group 4), and IR + 500 mg/kg MgSO4 (group 5). Testicular ischemia was achieved by 720 degrees torsion of the left testis for 2 hours. Then, detorsion was performed and reperfusion was induced. One hour after ischemia, three different doses of MgSO4 (125, 250, and 500 mg/kg) were administrated intraperitoneally. The sperm count, motility, mobility, and blood hematology were evaluated.

There was a significant increase in sperm count, motility, and mobility in groups treated with MgSO4 compared to the sham group (P<0.05). In addition, lymphocyte count in the sham group significantly increased compared to the IR group (P<0.05).

Generally, this study demonstrated that MgSO4 can improve sperm characteristics after testicular IR injury.