Evaluation of the Resistance of Some Indigenous Wheat Genotypes to Cereal Cyst Nematode, Heterodera Filipjevi

Cereal cyst nematode (CCN) is one of the most deleterious pathogens of cereal crops worldwide including Iran. The species Heterodera filipjevi is widely distributed in the fields of wheat. Growing the resistant cultivars is a suitable environmental friendly and cost-saving procedure for nematode control. In current research, the resistance of 13 indigenous wheat genotypes and seven common cultivars of bread wheat to H. filipjevi were investigated in a completely randomized design with four replicates, under greenhouse conditions. Regarding to identification of the cyst nematode used in this study, the collected specimen was diagnosed through classical and molecular methods. Based on morphological and morphometric characters of cyst and second stage juvenile, amplification of rDNA-ITS regions and restriction fragment length polymorphism (RFLP), the studied cereal cyst nematode was identified as H. filipjevi. According to the analysis of variance, the effect of total numbers of cysts and females on the cultivars was significant at 1% level. The cultivars of Bezostaya and Katae, showed the highest and lowest mean total population in soil and root, respectively. Bezostaya showed susceptible reaction, katae, Lines 84 and 85 were grouped as resistant whilst, the other cultivars and lines showed moderately resistance reactions.