Evaluation of the Association between the Risk of Breast Cancerand rs17065417 Polymorphism in the LIN28B Gene

LIN28B is one of the genes that have been shown to be involved in various cancers and diseases, such as cervical, neuroblastoma, and breast cancers. This gene is a key inhibitor of the let-7 miRNA and can inhibit let-7 expression. This study aimed to investigate the association between the risk of breast cancer and rs17065417 polymorphism in the LIN28B gene.

In this case-control study, genomic DNA was extracted from whole blood samples of 100 patients with breast cancer and 100 healthy individuals. The polymerase chain reaction (PCR) technique was used to amplify the region containing the polymorphic site. Finally, PCR products were sequenced and genotypes were determined using Chromas software.

Based on the results of the statistical test, regarding the AA genotype, the control (80%) and patient (73%) groups obtained the highest frequency. Moreover, the lowest frequencies in the AC and CC genotypes were reported in the control (8%) and patient groups (5%). In addition, the genotype AC associated with a 6-fold increased risk of breast cancer (P=0.005; OR=6.600; CI=1.763-24.714).

  It seems that there is a relationship between rs17065417 polymorphism and an increased risk of breast cancer in the LIN28B gene.