Effect of Continuous Exercise Training on Protein Levels of SIRT3 and OGG1 in the Liver Tissue of Male Wistar Rats

Sirtuin-3 (SIRT3) acetylates and activates the mitochondrial enzymes and helps to preserve mitochondrial integrity and prevents apoptosis through regulating Oxo-Guanine Glycosylase-1 (OGG-1). Resveratrol could stimulate SIRT3 and the athletes use this supplement to support the effects of training. This study aimed to evaluate the effect of continuous training and Resveratrol consumption on SIRT3 and OGG1 proteins in the liver tissue of male rats.

In total, 32 adult male rats were divided randomly into four equal groups of control (1. Con), Resveratrol supplement consumption (2.R), Continuous exercise training (3.C), and Continuous exercise+Resveratrol (4.C+R). Continuous exercise training was performed for 6 weeks (6 days per week) at 80% of VO2max on a treadmill.  In addition, groups 2 and 4 received 50 mg/kg body mass/day of Resveratrol. At the end of the protocol, the rats were anesthetized with   Ketamine/Xylazine, and their liver samples were removed to evaluate the protein levels of SIRT3 and OGG1 using an enzyme linked immunosorbent assay. The data were analyzed through a one-way Anova test. A pvalue less than 0.05 were considered statistically significant.

The results indicated that Resveratrol consumption was significantly increased the SIRT3 levels in the second group (i.e., R) (P=0.01) and the fourth group (i.e., C+R) (P=0.0001); moreover, it led to an increase in the OGG1 level in the fourth group (i.e., C+R) (P=0.0001); however, it had no effects on the OGG1 levels in the R group (P=0.91). In addition, SIRT3 and OGG1 levels were significantly increased in the third group (i.e., C) (P=0.0001), compared to the R group.

A combination of Resveratrol supplement with continuous exercise training would have probably more positive effects on SIRT3 and OGG1 levels, compared to the training or the consumption of the supplement alone; therefore, this supplement is recommended to be used with continuous exercises.