Performance of different levels of ZnO nanoparticles on the amount of antioxidant enzymes in the liver of Koi fish (Cyprinus carpio)

Advanced industrial applications of nanoparticles (NPs) increase the likelihood of their dissolution in aquatic ecosystems, and on the other hand, their use by aquatic organisms, before the disappearance of these particles, have been threaten the life of aquatic animals. Since the nanoparticles after induction change the physiological mechanisms of fish, the aim of this study was to investigate the effect of zinc oxide nanoparticles (0,1,2,4,8 mg/l) on the antioxidant enzymes of the liver in Koi (Cyprinus Carpio) in a period of 2 and 10 days and was based on static method. ZnO nanoparticles caused significant changes in hepatic enzymes(p<0.05). These changes in hepatic enzymes at the end of the 10th day included a decrease in superoxide dismutase levels (SOD) 92± 0.13, glutathione peroxidase (GSH-Px) 19.14 ± 27, catalase (CAT) 36.21± 0.23 (nmol/Min/mg prot-1) and an increase Malondialdehyde level (MDA) was 50.18± 0.97 (nmol mg prot-1) compared to the control group, which depended on the dose level and duration of exposure to the nanoparticles.These results indicate that zinc nanoparticles in the aqueous media with the studied concentrations increase oxidative stress and have inappropriate effects on the hepatic enzymes, and these parameters are subject to severe changes.