Effect of zinc oxide nanoparticles synthesized by aqueous extract of hyssopus officinalis on colon cancer cell line (HT-29) and normal cell line (Huvec), and also on proxytonin protein (PCT) in Invivo environment

Today, with the increasing rates of cancer, especially colon cancer, and due to the side effects of chemical drugs in the treatment of these diseases, more attention to safe drugs with less side effects is needed. This study aimed to evaluate the toxicity of nanoparticles of zinc oxide synthesized by green hyssop plant extracts method, on colon cancer cell line (HT29) and examined the effects of nanoparticles on calcitonin proxy blood protein

In this in vitro study, the MTT method was used to evaluate the toxicity of this nanoparticle at four concentrations (250, 125, 62.5, 31.2 μg/ml) against normal cells and colon cancer cells. In the in vivo environment, 20 adult male mice were randomly assigned into 3 experimental groups (n=5), 100, 200, 300 mg/kg of 20 nm oxide nanoparticles, orally, and the control group (without receiving nanoparticles). Finally, the mice were anesthetized by an ether and an autopsy and blood samples from the heart was taken to carry out the proxitonin protein test by a solvent cannula in the clinical laboratory.

These nanoparticles are capable of inhibiting cancer cells at low concentrations (62.5, 31.2 μg/ml) and IC50 is reported to be about 62.5 μg/ml (P<0.0001). The results of the PCT test as a result of treatment of mice showed a slight increase in proxytonin protein at a concentration of 300 mg/kg (P<0.0001).

By increasing the concentration of zinc oxide nanoparticles synthesized by the green method from the aqueous extract of the hyssop plant, its toxicity increases and causes a rise in the proxytonin protein in the blood.