The effect of endurance training and berberine chloride consumption on antioxidant enzymes in diabetic male Wistar rats heart tissue

Oxidative stress is involved in the pathogenesis of many diseases. Oxidative stress and antioxidant imbalance play a major role in the outbreak and development of diseases. This study aimed to investigate the effect of four weeks of endurance training and consumption of berberine chloride on cardiac tissue antioxidant enzymes in diabetic male Wistar rats.

In this experimental study, 40 adult male Wistar rats weighing approximately 200-250 g were randomly divided into 5 groups including healthy control group (without STZ injection, medication and exercise), diabetic control group (with STZ injection) , Diabetic Experimental Group+ Physical Exercise, Diabetic Experimental Group+ Berberine Intake (50 mg/kg), Diabetic Experimental Group+Physical Exercise+Berberine Intake. 24 hours after the last exercise session, the animals were isolated by intraperitoneal injection of ketamine anesthetic and heart tissue to measure the changes of the enzyme superoxide dismutase, catalase and glutathione peroxidase. Paired t-test and ANOVA were used to analyze the data using SPSS software (version 22) and at level α=0.05.

The results showed that there was a significant difference in the level of CAT, SOD and GPX activity in the diabetic group after the use of berburn supplementation and endurance training (P=0.001). Results showed that there was a significant difference between the amount of exercise effect in the diabetic+training+berberine group with diabetic+training group and diabetic+berbine group.

The results of this study showed that endurance training can have a significant and effective effect on the activity of anti oxidant enzymes in the heart tissue of diabetic rats.