In order to evaluate Germinability and seedling vigour associated with peanut seeds of the plants had been sprayed with methanol and mthylobacterium a laboratory research at 2 years was conducted in Islamic Azad University of Rasht. This study was conducted using standard germination test, cold and accelerated aging test. Each test using factorial experiment based on randomized complete block design with 16 treatments (bacteria populations mthylobacterium in unit of CFU in 4 levels, 106, 108 and 1012 and 4 levels of methanol 0, 10, 20 and 30 % v/v) in three replications. characteristics studied were: final germination percentage, seedling vigour, root dry weight, hypocotyl dry weight , shoot dry weight and seedling dry weight. The results showed that the effect of year was significant on all studied characteristics in all the tests. Bacteria population only significant effect on the final germination percentage in all the tests. The amount of methanol had significant effect on all studied characters in the three tests. Interaction between the bacterial population with amount of methanol only the final germination percentage in standard germination and cold tests had a significant effect. Comparison of means showed the highest final germination percentage in the population of bacteria 1012 CFU was observed in all the tests. Most of all investigated characteristics in all tests was 20 percent methanol.
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