Green Synthesis of Silver Nanoparticles with Allium paradoxum Extract and Evaluation of their Antibacterial Activities
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Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Background and purpose

Microbial resistance against antibiotics is increasingly growing, therefore, it is necessary to develop new remedies. Silver nanoparticles have significant antibacterial properties and synthesis of silver nanoparticles mediated by plant extracts could be considered as an alternative in treatment in various infections.

Materials and methods

In this study, silver nanoparticles were synthesized using Allium paradoxum extract. Optimum temperature, concentration, and also optimum pH for production of silver nanoparticles were established. Formation of the particles was investigated by UV-vis spectra, EDS, XRD, and SEM techniques. The MIC and MBC values of the particles against several gram-positive and gram-negative bacteria strains were determined.

Results

Optimum condition for synthesis of silver nanoparticles was at 85°C, pH 12, 5 mM concentration, and 30 minutes after adding the extract solution to silver nitrate solution. The particles remained stable until two months after synthesis. The approximate size of silver nanoparticles was 30 to 40 nanometer. Nanoparticles shows strong antibacterial effects against Pseudomonas aeruginosa, and moderate antibacterial activity against Staphylococcus aureus and Escherichia coli.

Conclusion

In this study, A. paradoxum was found as a good reducing and coating agent in green synthesis of silver nanoparticles. These particles showed strong antibacterial activities Therefore, they can have high potential for use in biological fields.

Language:
Persian
Published:
Journal of Mazandaran University of Medical Sciences, Volume:29 Issue: 182, 2020
Pages:
1 to 11
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