Experimental study of the effect of Zipmet on corneal ulcer healing due to burning in rabbit

This study was conducted to examine the effect of Zipmet on healing of corneal ulcer due to burning in rabbit. In this study, 20 adult healthy male NewZealand rabbits with an average weight of 2.5-3 kg were used. After general anesthesia, corneal ulcer in the left eye of the all animals was induced.The left eye of each rabbit was stained with 2 drops of tetraquine 0.05% . After 5 minutes, a circular filter paper of 6 mm in diameter, impregnated with 30 μL of a Sodium hydroxide 1 was placed in the cornea center for 60 seconds.After returning from anesthesia, the rabbits were randomly divided into 4 groups of 5 numbers. Then, in the first, second and third groups, Zipmet at 50, 100 and 200 mg / kg were gavaged, while the fourth group was gavaged with water for 21 days. After 21 days, corneal samples were taken and evaluated histopathologically. According to the histopathological findings, vascularization, inflammation and matrix edema of cornea significantly marked while the epithelium of cornea increased in metformin 200 mg / kg -treated rabbits compared to control group (P<0.05). Also, administration of Zipmet significantly improved the natural structure of cornea collagen fibers (P<0.05). Based on the results, it seems that Zipmet at dose of 200 mg / kg has useful effect on the healing process in rabbit’s corneal ulcer.