Effect of Tranylcypromine Drug on LH, FSH, Testosterone Hormones Concentration and Testicular Tissue Changes in Adult Male Rat

Tranylcypromine is a monoamine oxidase inhibitor (MAOI)-it is a nonselective and irreversible inhibitor of the enzyme monoamine oxidase (MAO). It is used as an antidepressant and anxuolytic agent in the clinical treatment of mood and anciety disorders, respectively. In this research, the effects of Tranylcypromine the serum concentrations of LH, FSH and Testosterone as well as changes in body weight and testicular tissue were studied in rats.

50 adult male Wistar rats with approximate weight of 200-250 gr were divided into 5 groups of 10 animals: the control ,sham group and three experimental groups receiving 40, 20 and 10 mg/ kg Tranylcypromine, respectively. The drug was orally administered for 21 days. Following wighting at the end of this period, blood samples were taken from each rats, centrifuged to obtain serum, and testis were removed,weighted and fixed to examine their possible histological changes. Serum samples were frozen at -20 Ԩ to be studied later. The serum cocentrations of LH, FSH and testosterone were measured by RIA. After tissue sectioning and staining with hematoxylin-eosin, testicular tissues were examined by light microscope. The SPSS and Excel software and ANOVA and Tukey tests were used for statistic analyze.

The results showed that 21 days consumption of maximum doses of Tranylcypromine, significantly increases concentrations of LH and FSH hormones and significantly reduce testosterone concentration in maximum doses and leydig cells in all three experimental groups (P≤0.05). In addition, no significant effect was observed in the body weight, testicular weight as well as Sertoli cells. Moreover, testicular tissue study indicates that 21 days of Tranylcypromine consumption significantly reduces spermatogony,spermatid , spermatocyte and thus, affects spermatogenesis.

Tranylcypromine drug reduced levels of testosterone and increase levels of LH and FSH hormones.