Effect of Single-Dose Methotrexate Treatment on Ovarian Reserve in Women with Ectopic Pregnancy Undergoing Infertility Treatment: A Single-Center Experience

The aim of this study was the evaluation of the impact of single-dose methotrexate (MTX) treatment on ovarian reserve in women with ectopic pregnancy undergoing infertility treatment in Iranian population.

This prospective cohort study was conducted between March 2015 and March of 2017 in Tehran General Women Hospital in Tehran, Iran. We enrolled 20 patients with ectopic pregnancy (EP) whose pregnancy happened during infertility treatment and they received a single–dose MTX (50 mg/m2) intramuscularly. Serum anti-mullerian hormone (AMH), 17 beta-estradiol (E2), luteinizing hormone (LH), follicle-stimulating hormone (FSH) as well as antral follicle count (AFC) on trans-vaginal ultrasonography were evaluated before and 8 weeks after administering MTX.

Mean±SD of AMH was not statistically significant before and after the administration of MTX (9.50±4.23 vs. 9.15±4.24, P=0.36). FSH, Estradiol and AFC changes were not statistically significant, while increasing the value of LH was significant (P=0.02).

Results indicated that single-dose MTX treatment had no effect on ovarian reserve in women with ectopic pregnancy. Further randomized controlled clinical trial study with larger sample size, multiple dosage of MTX, long-term follow up is suggested.