The Effect of Nitrogen and Phosphor Fertilizer Usage on Ta-PHR1, Ta-PHO2 Gene Expression, Yield and Related Traits in Bread Wheat

Phosphor is one of the most important element in wheat. It has crucial role in yield production and transfer energy process. Therefore, supplying of phosphors in order to yield consistency is necessarily. In order to investigate the effect of using phosphor and nitrogen fertilizer on Ta-PHR1 and Ta-PHO2 gene expiration as well as yield and related traits, experiment carried out by using factorial split plot format in randomized complete block design with three replications. Main plot was included factorial combination of two level of nitrogen fertilizer (0 and 150kg/ha) and two level of phosphor fertilizer (0 and 100kg/ha), sub plot was included four wheat genotypes (Morvarid variety and N80-19, T-67-60, T-65-7-1 promising lines). The yield and related traits were measured at final stage of maturity and gene expression was assessed at grain filing stage. The results showed the most yield and most related traits (distance between node, spike length, shoot length, fertile tiller number, total tiller number, seed number, seed diameter, seed length, 1000 seed weight) were reached at using both nitrogen and phosphor treatment. Also, Morvarid variety and N80-19 line have more yield in both combined treatment (using both nitrogen and phosphor fertilizer) and in control condition (using no fertilizer). The most gene expression of Ta-PHR1 was assessed on Morvarid variety and T-65-7-1 line respectively. Also, usage of fertilizer had positive and significant effect on all studied traits except seed diameter.