Effect of Ethyl Acetate and Aqueous Fractions of Ziziphus jujuba Extract on Biochemical and Hematological Parameters in Rat

Despite the numerous therapeutic effects of the Ziziphus jujuba (ZJ),its effect onbiochemical,hematological parameters are unknown. Therefore,in this study,the effect of ethylacetate (EA),aqueous (AQ) fractions of the ZJ fruit extract on biochemical,hematologicalparameters were explored. Material,

Thirty-six male Wistar rats randomly were dividedinto six groups as follows: (1) Control,(2) ZJ extract (200 mg,kg),(3,4) EA fraction of ZJ (EA150and EA300 mg,kg),and (5,6) AQ fraction of ZJ (AQ150,AQ300 mg,kg). Saline,ZJ extract,andits fractions were gavage once a day for 4 weeks. At the day of the experiment (28th day),one bloodsample was collected in citrated tube for the counting of the complete blood cell count calculation.The serum was used for biochemical parameters (cholesterol,triglyceride,and glucose concentrations)evaluation.

The results showed that the blood glucose,cholesterol,and triglyceride levels inall treated groups,especially in AQ groups,significantly decreased compared to the control (P < 0.01to P < 0.001) group. Furthermore,the number of red blood cell,white blood cell in treating ratswith ZJ extract,its AQ fraction significantly increased (P < 0.05),whereas the number of plateletssignificantly decreased (P < 0.05) compared to the control.

In conclusion,both AQ andEA fractions of ZJ have beneficial effects on biochemical,hematological parameters. Since theeffect of AQ fraction is higher than EA,it is suggested that these effects of ZJ mostly mediated bycompounds of ZJ that soluble in the polar solvent.