Isolation, molecular identification and optimization of amylase production of marine actinomycete (Acx1) from Shadegan estuary

Actinomycetes, Gram positive bacteria, are rich sources of bioactive compounds. Secondary metabolites of these bacteria are used as pharmaceutical compounds Enzymes are one of the most important secondary metabolites which act as bio-catalysts to perform reactions in bio-processes in an economical and environmentally-friendly manner. Enzyme production from actinomycetes makes significant facility in industrial and world economic. So, that it is becoming one of the economic indexes of developed countries. The goal of this research is isolation of actinomycetes from Shadegan estuary, optimization of bacterial growth and amylase production. Molecular identification was done by amplification of 16S rRNA gene using R1492 and F27 primers. Optimum growth condition was found at 6% NaCl concentration, 35˚C temperature and pH 8 . Maximum amylase activity in optimum condition was 80150 Unit (one Unit is amount of enzyme that release one microgram of glucose in a minute). The results showed that enzyme production is related to growth of bacteria conditions.