Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone Nanofiber

Through combining two synthetic and natural polymers, scaffolds can be developed for tissue engineering and regenerative medicine purposes.

In this work, CMC (20%) was grafted to PCL nanofibers using the cold atmospheric plasma of helium. The PCL scaffolds were exposed to CAP, and functional groups were developed on the PCL surface.

The results of FTIR confirmed CMC (20%) graft on PCL scaffold. The MTT assay showed a significant enhancement (p < 0.05) in the cell affinity and proliferation of ADSCs to CMC20%-graft-PCL scaffolds. After 14 days, bone differentiation was affirmed through alizarin red and calcium depositions.

Based on the results, the CMC20%-graft-PCL can support the proliferation of ADSCs and induce the differentiation into bone with longer culture time.