Effects of 6 weeks of aerobic training on the level of serum and tumour tissues ghrelin in mice with breast cancer

Ghrelin has a dual effect on breast cancer development. The aim of this study was to evaluate the effect of aerobic training on the level of serum and tumour tissues ghrelin in mice with breast cancer.

Breast cancer cells MC4-L2 were implanted to mice and they randomly categorized into two groups including control (n=8) and training (n=8) groups. Training group performed progressive aerobic training 5 days per week for 6 weeks on treadmill and control group didn’t any training. Tumor volume was measured by a digital caliper weekly. Finally, the mice were sacrificed; serum and tumor tissue were removed and immediately frozen and kept in -70°C. Assay of ghrelin was performed by ELISA kit with code number RAB0207. Independent sample t-test and repeated measure analysis of varicance were used for extraction of results at the significance level of p

The results of t-test showed that the level of ghrelin in tumor (p=0.02) and serum (p=0.002) were significantly lower and higher compared to the control group. In addition, the mice heart to weight ratio was significantly higher in the training group (p=0.001) than in the control group. The result of repeated measure ANOVA showed there were significant differences between the two groups in tumor volume and food intake (p=0.001).

According to the finding, aerobic training in tumor bearing mice have a benefits in reducing tumor volume, maintaining food intake and weight by modulating ghrelin levels in tumor tissue and serum.