Effect of Selenium Nanoparticles (Se NPs), in the Germination and Some Morphophysiological Characteristics of (Astragalus gossypinus Fisher) in MS Culture Medium

To investigate the effect of seeds priming of Astragalus gossypinus Fisher. with MS and selenium nanoparticles (SeNPs) solution in early stages of germination, a factorial experiment was conducted in a completely randomized design (CRD) with four replications in the Laboratory of Production and Duplication Center of Municipality of Kashan, in 2017. Experimental treatments consisted of priming with MS solution at 4 levels (0 as control, 0.01, 0.05 and 0.1 % w/v), and SeNPs in 4 levels (0 as control, 0.05, 0.1 and 0.2 %w/v) for 2 hours at 25 ° C. The results of the experiments indicated that MS, SeNPs solution and interaction of treatments were significant at the 1 % level on all studied traits, including of germination percentage, radicle length, plumule length, germination coefficient, relative water content (RWC), chlorophyll a, b and total chlorophyll content. The highest germination percentage, the content of chlorophyll a and b, and plumule length were obtained in application of combined MS solution 0.01% w/v with 0.2% w/v of SeNPs. On the other hand, application of these treatments alone had positive and significant effects on the studied traits. The use of priming methods, such as used methods in this research, and its effects on Astragalus gossypinus are innovations of this research.