Effect of dietary supplementation of Chlorella vulgaris on several physiological parameters of grey mullet, Mugil cephalus

The present study was investigated on effect of optimum dietary level of Chlorella vulgaris powder (CP) as a feeding supplement on various blood biochemical criterion (Cholesterol (CHO), triglyceride (TG), total protein (TP), glucose (GLU), and lysozyme) and digestive enzymatic activities (amylase, lipase and protease) of the grey mullet (Mugil cephalus L). Four experimental regimens were supplemented with CP at 0, 5, 10 and 15 g kg−1 diet (CP0, CP5, CP10 and CP15). Number of twelve pools (60-L) with three duplicates for analysis groups (n=10 per pool with initial weight average 14.95±2.01 g) and the control group were studied. Upon 60 days of the feeding trial, fish fed CP5 diet had lower serum CHO and TG levels than fish fed CP0, CP10 and CP15 diets (p<0.05). No considerable difference were found in GLU when comparing fish fed CP5 and CP10 diet (p>0.05). Most serum total protein and amylase, protease, lipase and lysozyme activities were observed in fish fed CP5. Also, fish fed CP10 and CP15 diets had higher digestive enzymatic activities, serum total protein and lysozyme activities than fish fed CP0 (p<0.05). The outcomes proved the inclusion of 5g chlorella powder dietary supplementation in the commercial regimen may improve the blood chemical responses and the activity of digestive enzyme in grey mullet.