Role of stratification and priming with arginine and gibberellic acid on germination character’s of Fritillaria raddeana

Fritillaria raddeana Regel is one of the Iranian-native bulbous plants well known for its medicinal and ornamental values. As regards germination and establishment are two key points in order to protect the natural populations of this species, it is important to study about propagation and domestication of F.raddeana. An experiment was conducted to study the status of seed dormancy to break it in a randomized completely design including 5 priming treatment with 7 replicate in 2015- 2016. Priming treatments were arginine; in concentrations of 5 and 10 mM), gibberellic acid; concentration of 250 and 500 mg per liter, and control (without priming). All events throughout germination process seed was recorded graphically for an image. The results showed that seed priming with arginine and gibberellic acid improved germination properties. Among all treatments, arginine 5 mM has greatest impact on the rate and percentage of germination as far as it increased percentage and rate germination by 1.5 and 2.5 fold in companion with control, respectively. However, there is no significant different between arginine 10 mM and two concentration of gibberellic acid in germination results. The study on the growth of the embryo showed that F.raddeana seeds have after-ripening, so that embryo size is third of its actual size in natural habitat that gradually developed with receiving cold treatment.