Mycoflora of hybrid sorghum seeds in line production areas of Khorasan Razavi

Mycoflora of hybrid sorghum seeds in line production areas of Khorasan Razavi Seed health analysis was carried out on 30 Sorghum bicolor cv. speed feed(line AS9) seed samples belonging to two growing seasons (2001-2004) which were routinely submitted by Feizabad Research Center, Khorasan Razavi provience to Seed and Plant Certification and Registration Institute, Iran. Applying simple blotter-, deep freezing blotter-, and agar plate- methods for detection of seedborne mycoflora revealed the presence of Fusarium verticilloides, Bipolaris sorghicola, Cladosporium sp., Alternaria sp., Penicillium spp.,and Cephalosporium acremonium in seed samples at the rates of 0.5, 4.5, 16, 19.25, 21 and 0.75 percent, respectively. Using washing test, teliospores of Sphacelotheca cruenta and Sporisorium sorghi at the rates of 12 and 42 teliospores/Kg seed were detectedon the basis of teliospore morphology.