Comparison of antimicrobial effects of several plant extracts with silversulfadiazine in the treatment of burns caused by Pseudomonas aeruginosa in vitro and in vivo

Infectious diseases are one of the most common diseases in the world, including Pseudomonas aeruginosa. In burn wounds, Pseudomonas aeruginosa is used to treat burns, but a number of resistances have been created, which has led to the use of plants to treat diseases. The aim of this study was to compare the effects of antimicrobial and healing marigold extract, eucalyptus, walnut, jujube, turpentine oil with silver sulfadiazine to create an effective agent in the treatment of burns is due to Pseudomonas aeruginosa.

Alcoholic and ethanolic extracts of walnut and jujube leaves were prepared in the laboratory and Eucalyptus oil, turpentine oil and marigold extracts were prepared for ready and we also used silver sulfadiazine ointment. In the next step, the MIC and MBC extracts were determined. In the animal model, the burn was initially burned and then infected by the bacterium Pseudomonas aeruginosa. Then the silver sulfadiazine ointment was used alone and in combination with each of the extracts separately and one gram and for three consecutive days, was injected into the wound of mice and The number of bacterial colonies was counted after the culture on the Muller Hinton Agar and culture Nutriant Agar.

MIC and MBC of Extracts of Marigold 5.25 and 10.50 mg/ml, Eucalyptus 1.75 and 3.50 mg/ml, Alcoholic Extract of Walnut 59.0875 and 118.175 mg/ml, Estonian Extract of Walnut 8.375 and 16.75 mg/ml, Alcoholic extracts of jujube 39.875 and 79.75 mg/ml, Estonia extracts of jujube 8.625 and 17.25 mg/ml and Turpentine 8.375 and 16.75 mg/ml. The study of mouse model confirmed the effect of extracts with silver sulfadiazine against Pseudomonas aeruginosa. The acetone extract of walnut leaf, the acetone and alcoholic extract of jujube, as well as the eucalyptus extract, reduced the number of Pseudomonas aeruginosa bacteria and had better antimicrobial activity against Pseudomonas aeruginosa than other groups.

Alcoholic juices and Estonian jujube have a better effect than other groups.