Determination of nutritive values of the green and black tea waste in ruminant nutrition using in vitro gas production technique

The aim of this study was to determine of nutritive value of green and black tea waste, and determination of metabolizable energy (ME) and net energy for lactation (NEL) using in vitro gas production method. The obtained data was completely randomized design was analyzed using SAS procedures. The values of crude protein, crude ash, ether extract, neutral detergent fiber and acid detergent fiber in green tea waste were 13.7, 3.7, 2, 44.6 and 31.7 %DM, also for black tea waste were 14.8, 4.7, 2.4, 39.6 and 27.1 %DM, respectively. The value of total phenol and extractable tannin for green tea waste were 20.3 and 18.7 %DM, and for black tea waste were 18.7 and 12.7 %DM, respectively. After 96 h of incubation, black tea waste and alfalfa had the highest and lowest gas production volume among the test feeds (p<0.05). The gas production potential amounts of the green and black tea waste and alfalfa hay were obtained 284.2, 294 and 261.9 ml/g DM, respectively. Rate of gas production for green and black tea waste and alfalfa hay were 0.074, 0.074 and 0.072 (h-1), respectively. Black tea waste had the greatest estimated metabolizable energy and net energy for lactation (9.45 and 5.77 MJ/Kg DM) among feedstuffs. These results indicated that black and green tea waste had a nutritive value higher than last bloom alfalfa and can be used as a part of ruminants ration.