Effect of fungul pathogen Leptosphaeria maculans at biotrophic stage on canola metabolic profile cultivar Hyola 401 in a compatible interaction

Recent improvements in instrumentation and data mining have made it possible to investigate the effects of various stressors on primary and secondary metabolisms. In this study, in order to study mechanisms of pathogenesis and identify the pathogenesis-related metabolic pathways, changes in metabolic profiles resulting from a compatible interaction between canola cultivar (Hyola 401) and Leptosphaeria maculans (anamorph Phoma lingam) (Phkv102 isolate) were investigated by GC-MS chromatography using the polar fraction of plant extract. Under completely controlled conditions, 14 days after planting, when cotyledons were completely unfolded, the plants were inoculated by 107 spores per ml suspensions and water (control) by drop-wound method, respectively. The tissue samples were taken 48 hours after inoculation and were shock frozen immediately. Metabolites were extracted using methanol as solvent, identified and quantified with GC-MS and subjected to statistical analyses. Results indicated that 70 metabolites showed significant difference over their controls at P≤0.05, which is caused by changes in 28 metabolic pathways. Infection of canola cultivar Hyola 401 to P. lingam resulted in change in pathways related to the host cell wall, phenolic compounds, alkaloids and terpenoids biosyntesis, energy generator, hormone biosynthesis, signal transduction.The abundance of a number of sugars was also decreased, suggesting the crucial role of these carbohydrates in pathogenesis in an incompatible interaction of  P. lingam and a susceptible cultivar.