Study of Cellulase Enzymes Activity in Rumen Fluid of Fattening Slaughtered Goat Kids

An experiment was conducted to investigate the activity of carboxymethylcellulase and microcrystalline-cellulase (Nonomol/min) ruminal hydrolytic enzymes from different fractions of particulate material, extra cellular or cellular. For this purpose, 15 rumen fluids of slaughtered fatteninig Najdi goat kids at the age of 6 months with an average of 28 kg were sampled in 3 treatments and 5 replicates. The experimental diets included 0, 10, 20 percent olive cake was replaced by barley. The results showed that the highest enzyme activity for both enzymes was in the fractions of particulate material in the goat kids fed diet was 20 percent olive cake (P<0.05). Also, the highest total activity of microcrystalline cellulase and carboxymethyl cellulase enzymes was observed in the goats fed with 20% olive cake (nmol / min) compared with other treatments (P<0.0001). Moreover, carboxymethyl-cellulase in particulate material, cellular, extra celluar and total activity where higher than microcrystalline-cellulase. The ruminal pH of goats decreased significantly with the increase in the olive cake in the diet (P <0.0001). The lowest PH in goat kids fed with 20% olive cake was seen compared to other treatments. Overall, the results of this study showed that with increasing levels of olive cake in the diet, the activity of cellulolytic enzymes increased in all three fraction of the rumen contents. The activity of enzymes in particulate material fraction was the highest in all treatments and the extracellular fractions where lowest (P<0.05).