There is demand for the production of new and effective antimicrobial compounds because the pathogenic bacteria evoluted and resistance to antibiotics Butanol, Methanol and Aceton extracts of Peronia verruculata were obtained and the antibacterial effects of two bacteria, Staphylococcus aureous (gram-positive) and Escherichi coli (gram negative) were determined using MIC and MBC methods. Turbidity and transparency of microtubes containing different amounts of butanol extract showed that the minimum inhibitory concentration (MIC) of E. coli was 600 μg/μl and 800 μg/μl of Staphylococcus aureus. The growth status of E.coli in Muller Hinton Agar Plate at different concentrations of butanol extract showed a minimum bacterial concentration (MBC) of 800 μg/μl and for Staphylococcus aureus 100 μg/μl. Aceton and methanolic extracts of Peronia verrculata to 1000 μg/μl did not inhibit bacterial growth.
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